Fundamentals of Biochemistry is a comprehensive textbook designed to bridge the gap between fundamental concepts and practical applications in the field of biochemistry. Unlike many texts that focus solely on basic or applied aspects, this book integrates both, making it particularly valuable for undergraduate students in life sciences, agriculture, veterinary, and related fields. Drawing on years of teaching experience, the authors present complex biochemical topics in a clear and accessible manner, aiming to dispel misconceptions about the subject’s relevance and highlight its significance across numerous scientific and technological domains. With carefully structured content and an emphasis on ease of understanding, the book serves as an essential resource for students seeking a solid and holistic grasp of biochemistry. Additionally, the author encourages feedback to further enhance future editions, demonstrating a commitment to continuous improvement and academic excellence.

Theory

1. Introduction to Biochemistry
2. Chemical Foundations of Life
3. Different Types of Molecular Bonds
4. pH and Buffers
5. Carbohydrates
6. Amino Acids and Proteins
7. Lipids
8. Nucleotides and Nucleic Acids

Practicals

1. Qualitative Analysis of Carbohydrates
2. Quantitative Estimation of Glucose by Somogyi-Nelson Method
3. Estimation of Total Sugar By Dubois Method
4. Estimation of Reducing Sugars By DNS (Dinitrosalicylic) Method
5. Qualitative Analysis of Proteins and Amino Acids
6. Estimation of Protein By Lowry’s Method
7. Determination of Iodine Number of a Fat Sample
8. Determination of Saponification Value of Lipids
9. Estimation of Titrable Acidity
10. Estimation of Antioxidant Activity By DPPH Method
11. Estimation of Total Soluble Solids