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Plant Tissue Culture: Totipotency To Transgenic

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ISBN: 9788177544671
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Fundamental and recent trends in research activities and application of latest techniques have been taken in to account. The book has been divided in two parts; Part-I comprises Fundamental Techniques and Part- II comprises Molecular Techniques and Applications. The former describes basics of plant tissue culture through different chapters, such as Laboratory organization, Sterilization Technique, Media Preparation, Embryo Culture, Somatic Embryogenesis, Haploid Culture, Endosperm Culture, Protoplast Culture and Somatic Hybridization, In vitro Pollination and Fertilization, Somaclonal Variation, Micropropagation and Acclimatization. The Part-II discusses Genetic Transformation, Hairy Root Culture, Enhancement of Secondary Metabolites, Role of Metal as Elicitors, Molecular Markers, Genetic Fidelity, Microarray, Bioinformatics and Nanotechnology. A comprehensive Glossary, Subject Index and the exhaustive references at the end of chapters have made the book more useful and reader-friendly.

The chapters are very original as contributors have authority on the subjects. All the chapters have been discussed in every detail, starting from historical background to recent

information/techniques and applications, in lucid language with update references till 2011. The results and important milestones are depicted in the form of tables, histograms, sketches, flow diagrams and with original photographs.

The key feature of the present book is the introduction of some of the new chapters; Genetic Fidelity, Role of Microarray, Bioinformatics and Nanotechnology.

Sharma HP

555

Table of Contents..

Plant Tissue Culture: An Overview
H. P. Sharma
Tissue Culture Laboratory Organization
V. K. Shahi and Shailesh Kumar
Plant Tissue Culture Media
Dr. J. V. V. Dogra
Sterilization Techniques in Plant Tissue Culture
Amarendra Narayan Misra and Meena Misra
Somatic Embryogenesis and its Application
G. R. Rout and C. Pradhan
In Vitro Organogenesis: Induction, Regulation and Applications
G. S. Shekhawat and Sonali Jana
Synthetic Seed
Santi Lata Sahoo, Jyoti Ranjan Rout and Satyajit Kanungo
Haploid Plant: Production, Prospective and Application
Kambaska Kumar Behera, Biswanath Bhunia and H. P. Sharma
Endosperm Tissue Culture: Technique and its Potential Application in Plant Improvement
Nishi Kumari
In Vitro Pollination and Fertilization- A Recent Update
Veena Agrawal and Kuldeep Shaarma
Embryo Culture: A Technical Approach to Overcome Hybrid Inviability
Mohammad Anis, Shahnaz Parveen, Anushi. A. Jahan and Ibrahim M. Aref
Protoplast Culture, Fusion and Somatic Hybridization
Y.K. Bansal and Shikha Bansal
Somaclonal Variation
Kavindra Nath Tiwari, Shikha Suman, Jayanti Singh and Pratima Kapur
Agrobacterium tumefaciens Mediated Genetic Transformation
Prof. A. Sadanandam Dr. A. Venkateshwar Rao and Dr. T. Christopher
Transgenic Banana: Challenges and Opportunities
V. A. Bapat and T.R Ganapathi, U. K. S. Shekhawat
Plastid Transformation in Higher Plants
A. Sadanandam, K. Venugopal Rao, P. Venkataiah, A. Venkateshwar Rao, and T.Christopher
Plant Propagation through In Vitro Production of Storage Organs
Dr. C. K. John
Micropropagation
K. Jaganmohan Reddy and M. Venkateshwarlu
Secondary Plant Products: Therapeutic Potential and Production through Plant Tissue Culture
Kishan G. Ramawat and Shaily Goyal, Jaya Arora
Transgenic Hairy Roots: Development and Applications
Sheela Chandra, M. M. Sharma
Role of Plant Tissue Culture in Conservation of Plant Genetic Resources
A. K. Sharma, Kavita Arora, Meena Sharma and Madhu Jain
Role of Metal Ions as Potential Abiotic Elicitors of Secondary Metabolites in Plant Cell, Tissue and Organ Culture Techniques
Bhumi Nath Tripathi and Vijeta Singh
Biogenic Synthesis of Metal Nanoparticles Using Plants
Dr. Rajesh Thakur
Bioinformatics in Relation to Plant Biotechnology
Gaurab Gangopadhyay and Sangita Basu Gangopadhyay
Applications of Molecular Markers in Plant Tissue Culture
A. K. Misra and Suman Kumaria
Assessment of Genetic Fidelity of the Tissue Culture Clones
Gaurab Gangopadhyay and Kalyan K Mukherjee
Role of Microarray in Plant Tissue Culture
Amla Batra, Abhijeet Singh, Madan Mohan Sharma and Deevan Julfikar Ali
Acclimatization of Tissue Cultured Plantlets
Madhuparna Banerjee
Plant Cell Tissue Culture and its Industrial Application
H. P. Sharma